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Scratch wound migration assays were performed as described previously (Pinco et al., 2002). As described previously (Pinco et al., 2002), green fluorescent protein (GFP)-tagged α4 integrin is functionally normal. GST-tagged proteins containing the disintegrin domains of ADAM12 (wild type and mutant), ADAM19 and ADAM33 were produced exactly as described previously (Tomczuk et al., 2003). Sequences encoding the disintegrin domains of mouse ADAM19 (residues G417-G504) or mouse ADAM 33 (residues P418-G505) in frame with a terminal 6Xhistidine tag were generated by PCR. ADAM12 disintegrin loop mutants (R481A, D488A, and E491A), were generated using a site-directed mutagenesis kit (Stratagene), using the 6XHis-tagged ADAM12 disintegrin domain as the template.
ADAM12 Inhibits α4β1- but Not α5β1 Integrin-mediated Cell Migration on Fibronectin
ADAM12 is phylogenetically related to Xenopus ADAM13, which has a documented role in cranial neural crest (CNC) cell migration (Alfandari et al., 2001). To date, only a few studies have addressed the possible role of ADAM-integrin interactions using full-length ADAM proteins in cell biological settings. Integrins often work in cooperation with syndecans, transmembrane proteoglycans that foster cell adhesion, stress fiber formation, cell migration, and invasion (Woods and Couchman, 2000; Beauvais and Rapraeger, 2004). Pinco support runs 24/7 to help with account, payments, bonuses, and technical issues. The site follows licensing standards, privacy compliance, and responsible gaming rules applicable to Canada. Promote the brand to a Canada audience and earn revenue share on referred players.
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